Frequently Asked Questions
What does this service cost?
Pricing information can be found here.
How long does it take to receive results after submitting samples?
Results are typically delivered within 2 weeks.
Are there any special shipping requirements for samples?
Samples should be shipped frozen on dry ice or at 2-8 Deg on cold packs for overnight delivery.
What is the minimum volume required per sample?
100 ul is preferred if IgG and IgM are run. 50 ul is acceptable if only IgG is run.
Do I need to dilute my samples?
No, samples should not be diluted. We dilute the sample 1:25 in the assay.
My samples are from SARS-CoV-2 infected individuals, do I need to de-activate the samples prior to shipping?
No, deactivation of the sample is not required prior to shipping.
Do you get quantitative information about antibodies? Do you get affinity of binding?
An enrichment value is calculated for each peptide epitope that is similar to an antibody titer. The combination of antibody affinity and concentration both contribute to this value.
How reproducible is your data?
In precision studies, the CVs for serology panels are generally <10%.
Is Serimmune able to accommodate a request for specific amino acid variants that I want to evaluate?
This is a custom service, but we can look at epitopes against arbitrary strains or variants.
How is this platform different from other available platforms?SERA vs. Antibody sequencing
Antibody sequencing does not provide information on the functional targets of the antibody, it only provides the sequence and quantity of antibody species.Array-based technologies
Array-based technologies and other technologies such as Virscan focus on specific proteomes or meta-proteomes. Because of the random nature of the SERA library, every sample processed can be leveraged for specificity and cross-reactivity analyses. New strains or variants can be analyzed without redesign of the library.SERA vs. Peptide arrays
Antigen based peptide arrays are limited to the exact linear amino acid sequences contained in the proteome. By contrast, SERA utilizes a random peptide library that can capture linear epitopes as well as non-mapping, conformational epitopes.SERA vs. Protein arrays
Protein arrays utilize whole proteins that are very expensive to develop and may exhibit lower specificity compared to peptide-based antibody detection.